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We review different methods to address the considerable challenges in modeling the binding of a short yet highly flexible peptide to its partner.
To investigate this conflicting evidence, we probed the direct interaction of a peptide derived from the C-terminal tail of FtsZ with ZapC by ITC (isothermal titration calorimetry) and obtained a K D of 32 μM, as expected for binding of a short, flexible peptide (Fig.2A and B).
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Generally, transcription factors regulate the expression of the target gene positively or negatively by binding to a short sequence of nucleotides called a transcription factor binding site (or motif) in the upstream region of their start point of transcription.
Found in all animal and plant cells, as well as in viral genomes, miRNAs act as inhibitors of protein translation by binding to a short six-nucleotide region within the 3′-untranslated region (3′-UTR) of their target mRNAs (Bartel 2004; Bartel and Chen 2004).
The cocaine RNA aptamer has been proposed as a means of constructing such a sensor, because it has the binding structure of a short hairpin flanked by two single-stranded regions [ 150].
The tilted hybrid conformation may compensate for the reduced binding affinity of a shorter hybrid, thus assisting in the formation of early transcripts.
A motif is a generalized representation of a binding site: a short region on the DNA, characterised by its sequence.
All the other hits, which showed recognizable sequence similarity to the N-terminal NAD(P -binding domain of Tic62, lack the C-terminal module (residues 387–534) resP -bindingor the FNR bindomainnd represent a shofTic62sion of the Tic62 protein.
Because someone like this can seem like a woman's prince charming, Dr. Schwartz cautions every woman to never enter into a serious relationship -- especially not one that is legally binding -- in a short amount of time!
DUESs themselves resemble sequence families that interact with sequence-specific DNA-binding proteins (e.g., CRP, LexA and other pro- and eukaryotic transcription factor-binding DNAs); which typically consist of a short conserved core extended by less conserved regions clustering around a consensus sequence without exactly reproducing it (see [63] [65]).
Like PDGF receptors, each of the ErbB receptors consists of an extracellular ligand-binding domain, a short membrane-spanning region and a cytoplasmic region possessing tyrosine kinase enzymatic activity.
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