Sentence examples for binding of a secondary from inspiring English sources

Briefly, depletion of CD4-negative T cells was achieved by incubation of peripheral blood mononuclear cells with a cocktail of monoclonal antibodies directed against non-T cells and against CD4-negative T cells, followed by binding of a secondary microbead-coupled antibody to bind to the primary antibodies.

Subsequent binding of specific antibodies followed by binding of a secondary antibody horseradish peroxidase (HRP) complex then allows quantitative evaluation of the analyte using tetramethylbenzidine as the substrate for HRP.

It is also possible that an unknown primary receptor is employed or that binding of a secondary receptor is required for successful viral attachment and entry; or a mix of both.

The graph shows a quantitation of the signal produced by the binding of a fluorescently labeled secondary antibody to the cleaved β domain on the Western blot.

This is not a result of oligonucleotide binding at a secondary site as the same signals are observed regardless of input oligonucleotides and this pattern disappeared when a different batch of RNA is used as shown in blue.

Intracellular localization, inhibition of in vitro transcription, and DNA binding suggest a secondary mode of action for the peptide, hinting at possible intracellular targets.

This would mean that IAP binding is a secondary function of these proteins and they may route through other pathways of apoptosis induction where caspase activity is only auxiliary to their apoptogenic potential.

Isothermal titration calorimetric data suggested a simple binding for G1, whereas a biphasic binding was evident for G4 and G7 with an initial exothermic binding and a secondary endothermic formation of larger dendriplex aggregates, followed by agglomeration.

After binding of an species-specific secondary antibody coupled to horseradish peroxidase, proteins were visualized by SuperSignal West Dura Extended Duration Substrate (Thermo Scientific, Waltham, MA, USA) and developed with LAS-3000 (FuJapanaccordingording to the instructions of the manufacturer.

We used a logistic regression to evaluate how well the propensity for nucleosome binding and co-occurrence of a secondary motif identify which canonical motifs are bound in vivo.

As the HAdV-A31 fiber shaft is shorter and probably less flexible than the fiber shaft of HAdV-A12, a functional binding of the secondary cellular receptor to the RGD motif of protein IX instead of the RGD motif in the penton base may be possible or even preferred.