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However, they failed to demonstrate binding of a recombinant IZUMO1 peptides with the eggs.
We could demonstrate direct binding of a recombinant fragment of C. elegans agrin to purified vertebrate α-DG by a membrane overlay assay.
A fluorescence microscopy-based in vitro assay was developed to screen compound libraries for molecules that prevented the binding of a recombinant human Ndc80 kinetochore complex to taxol-stabilized microtubules.
This cell-free system measures the ability of a compound to enhance the binding of a recombinant PPARα LBD to a PGC1α co-activator peptide, as measured by an increase in TR-FRET signal.
Here, we have characterized the three-dimensional structure and DNA binding of a recombinant human GINS complex.
The activation state of endogenous CHO cell α5β1 was assessed by measuring the binding of a recombinant soluble integrin-binding fragment of fibronectin (FN9 11) in three-colour flow cytometric assays as described earlier (Tadokoro et al, 2003; Calderwood et al, 2004).
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In this work, the binding kinetics of a recombinant mAb and its single-chain antibody homolog, single-chain variable fragment (scFv), was analyzed by SPR.
As shown in Figure 2, binding of a pure recombinant HMGA1 isoform to these probes produced a protein-DNA complex that was recognized by the anti-HMGA1-specific antibody and was supershifted to a slower-migrating form.
DOI: http://dx.doi.org/10.7554/eLife.03674.010 To assess the role of the intermolecular zinc finger in mediating PER CRY association, we first tested the CRY2-binding activity of a recombinant mutant PER2-CBD, which lacks the CXXC motif.
GLUT-1 was measured through binding to its ligand, the receptor binding domain (RBD) of a recombinant glycoprotein from the human T lymphotrophic virus (HTLV) fused to eGFP (HRBD-eGFP) (Montel-Hagen et al., 2008).
It was strange that a previous study by detecting of binding a recombinant human TNFRSF4-containing chimeric molecule did not observe TNFSF4 expression on any population in peripheral blood at a significant level.
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