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For this, we immobilised various quinolone derivatives on the sensor chip and tested binding of a range of different antibodies (polyclonal and one engineered antibody) in the presence and absence of free (fluoro quinolones.
These antibodies inhibited the binding of a range of clinical isolates from East and West Africa, although not all [54].
It seems possible that binding of a range of substrate conformations could be facilitated by the variety of orientations presented by the various R states.
Ligand-binding studies revealed high-affinity binding of sialic acid to NanU (Kd ~400 nM) from two Bacteroidetes species as well as binding of a range of sialic acid analogues.
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Binding pattern included binding to a range of CII fragments in the region of 25 to 100 kDa; and high-molecular-weight aggregates (higher than 250 kDa).
The common variant p.R279C resides within a PXXP motif, a functional motif in several proteins that confers binding to a range of domains, including SH2 and SH3 (22); analysis of p.R279C showed a subtle tendency of disorder-to-order transition.
Moreover, although plg displays widespread cell-surface binding to a range of putative receptors, it is the uPA-co-localised fraction of plg that is readily activated to pln [ 28].
C. jejuni 11168 showed significant binding to a range of fucosylated glycans.
The anti-BoNT sdAb were also tested for their binding to a range of BoNT subtypes using the various toxins as wells as their toxoids immobilized on microspheres.
These proteins contribute to pathogenicity by binding to a range of host factors mediating adhesion, invasion and resistance to the complement response.
Extracellular HMGB1 activates macrophages and DCs [ 135, 136 ] and burst neutrophil recruitment [ 137 ] by binding to a range of receptors, including TLR2, TLR4, and RAGE (Receptor for Advanced Glycation Endproducts) [ 135 ].
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