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(ii) Nuclear localization of Nrf2 coincides with increased DNA binding of a putative Nrf2/MafK heterodimer to its cognate cis-regulatory site, i.e., the antioxidant-responsive element (ARE).
The Q allele (A-SNP) seems to abrogate the binding of a putative repressor (van Laere et al, 2003).
This mechanism may imply that hypermethylation in a silencer region could determine an increase in gene expression by preventing the binding of a putative repressor.
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RCAS1 induces apoptosis of lymphocytes by binding to a putative RCAS1 receptor (Nakashima et al, 1999).
However, NPA functions as a general inhibitor of secretory processes (by binding to a putative NPA-binding protein), associated with the auxin efflux carrier site [ 31, 35].
CysB binding sites in proteobacteria [ 9, 10] differ considerably from binding sites of a putative factor that we predicted for cysT and cysA.
Recently, we characterized the binding of locostatin, a putative RKIP inhibitor, to the RKIP pocket [27].
In structure-based drug design, accurate crystal structure determination of protein ligand complexes is of utmost importance in order to elucidate the binding characteristics of a putative lead to a given target.
Two components, a permease and an ATP binding protein of a putative ribose ABC transport system (BOV_A0936-BOV_A0937) are pseudogenes in B. ovis, suggesting that the inability of B. ovis to utilize ribose as a sole carbon source may be the result of a reduced capacity to take up ribose from the growth medium.
Disruption of the P-type ATPase resulted in a non-vectored phenotype [ 23], whereas disruption of the solute binding protein of a putative glucose ABC-transporter led to a reduced level of transmission [ 27].
Interestingly, analysis of the Wnt7b promoter showed that deletion of the region between -1,005 bp and -829 bp relative to the second transcription start site significantly increased the basal transcription activity of a Wnt7b-luciferase reporter [ 30], suggesting that the binding element of a putative transcription inhibitor was deleted in this construct.
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