Slowing the photocycle with a targeted mutation may lead to photocontrol of K-helix binding by a monomeric form of the protein.
Based on the "gatekeeper phosphorylation" concept [46], one could speculate that high-affinity binding of one monomeric subunit of a 14-3-3ζ 14-3-3ζ 14-3-3ζLESdimerif in L1 could be toe prerequisithefoRSLESDing of a second 14-3-3 motiferin subunit to a second, L1wer-affinity site in L1.
These immunocytokines comprise of an IgG with high-affinity, bivalent binding to CEA or FAP, an Fc part with abolished FcγR binding, and a monomeric IL-2 variant (IL2v) binding only to IL2-Rβγ (abrogation of CD25 binding).
However, AChE binding to these two regions of a monomeric PrP or fibrillar PrP protomer appears to be mutually exclusive and, thus, only one of the two sub-sites in the PrP N-terminus can be occupied by AChE without any steric hindrance.
This work involved the use of computational methods to introduce a metal-binding site onto the surface of a monomeric protein to direct the formation of a dimer.
Although we noticed somewhat reduced dimerization of SYWT, no significant changes were observable for lipid raft binding of the monomeric forms upon combined expression.
This is for the first time we show the range of cellular changes and responses that take place immediately after the binding of the monomeric form of staphylococcal α-hemolysin.
This is indicative of weak binding of the monomeric unfolded mutant UBA to ubiquitin, consistent with pull-down experiments.
It is likely that this conformation is not maintained within the monomeric form of N-CEACAM1 which, as confirmed by the binding of the monomeric V39A mutant (Watt et al, 2001), is the active form for binding to UspA1 (Supplementary data).
The dimeric B-ZIP protein CREB1 (Vinson et al. 2002) enhances binding of the monomeric ETS protein GABPα (Batchelor et al. 1998; Garvie et al. 2001; Hollenhorst et al. 2011a) to the ETS↔CRE motif only when the two motifs are spaced in the configuration shown above (Chatterjee et al. 2011).
