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The system automatically monitors the binding of a fluorescent dye to double-strand DNA by real-time detection of the fluorescence present during each cycle of PCR amplification.
The system automatically monitors binding of a fluorescent dye to double-strand DNA by real-time detection of the fluorescence emitted during each cycle of PCR amplification.
To quantify the binding of a fluorescent probe to a specific tumor receptor, determining the pharmacokinetics of the intensity contrast between tumor and contralateral site is required.
The aFGF Cys2 mutant was recombinantly synthesized by substituting the second amino acid with a reactive cysteine whose sulfhydryl group's side chain reactivity facilitated the covalent binding of a fluorescent probe as a thiolyte monobromobimane.
In one type of method, certain ligand-binding polypeptide tags were fused with target proteins in recombinant fusion proteins to achieve non-covalent binding of a fluorescent ligand [2], a labeled polypeptide ligand [3], or other small molecule ligands [4] [6].
Similarly, the binding of a fluorescent marker for hypoxic cells, the nitrofuran AF-2, was markedly enhanced by WR-2721 andition, andecreaseded by BSO-induced thiol depletion.
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To determine the rate of SN-induced apoptosis in these cells, the amount of activated caspases (caspase-1, -3, -4, -5, -6, -7, -8 and -9) was measured (binding of a red fluorescent-labelled inhibitor to active caspases), as the expression of GFP interfered with the annexin V-FITC/PI assay otherwise used.
EBNA1 DNA binding domain was purified to near homogeneity and capable of binding a fluorescent tagged DNA hairpin containing a consensus EBNA1 binding site with ∼50 nM affinity (data not shown).
We have achieved the goal of binding a fluorescent dye to the CXCR4 chemokine receptor via a small molecule antagonist conjugate.
To examine whether inhibition of catalytic activity resulted from the inhibition of substrate binding, a fluorescent assay for As III) binding was employed.
The presence of chitin in the pharynx has been confirmed by the binding of a chitin specific fluorescent protein (Zhang et al., 2005).
More suggestions(15)
binding of a molecular
binding of a basic
binding of a ferrocene
binding of a monomeric
binding of a different
binding of a certain
binding of a second
binding of a fatty
binding of a respective
binding of a biotinylated-BH4-BCL2
binding of a PPARα-RXRα
binding of a nucleotide
binding of a forthcoming
binding of a cellular
binding of a single
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