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Long-distance transport of the signals of neurotrophic factors, and many other growth factors that employ receptor tyrosine kinases (RTK), routinely features the binding of a factor to its specific RTK followed by endocytosis and retrograde axonal delivery within the endo/lysosomal system to the cell body.
H3S10 phosphorylation was put forward as part of a 'binary switch' mechanism of the 'methyl/phos' type: phosphorylation adjacent to a methyl mark leads to induced loss of methyl-based binding of a factor or complex [ 59, 81].
As a chromatin remodeler is recruited to the target genes by its factors, the binding of a factor may decide the nucleosome positions in the active and repressed state of a gene region [ 14].
These experiments indicate that the increase in G6PD activity induced by DSBs is retained after the pull down and might be due to a stable modification of G6PD such as a post-translational modification of the enzyme or the binding of a factor that increases its activity.
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A transcriptional regulatory process that involves either antiparallel binding of two copies of a transcription factor, or binding of a homodimeric factor with antiparallel binding sites, would require an inverted repeat-binding site.
Similarly, any transcriptional regulatory activity that requires either antiparallel binding of two copies of a transcription factor, or binding of a homodimeric factor with antiparallel binding sites, would require an IR.
Each element of the matrix indicates the binding of a transcription factor to an H2H gene pair. 1 represents a transcription factor binding to an H2H pair, 0 represents a transcription factor not binding to an H2H pair.
Oct-1 might activate the epithelial- specific HPV16 enhancer by stabilizing the binding of a nuclear factor to the composite element, which in turn results in higher levels of enhancer activity[ 33].
On the other hand, the formation of certain combinations of histone modifications around transcription factor consensus sequences precedes, and therefore may direct, the binding of a transcription factor (Guccione et al., 2006).
ChIP assay is used for detecting the binding of a transcription factor with its response elements on gene promoters or regulatory sequences in tissue or cell lysates under real biological situation.
TGF-β-mediated activation of the COL1A2 gene is triggered by binding of a transcription factor complex comprising SP1, p300, and Smad2/3 to the SP1 sites in its promoter region [ 9].
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