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Notably, Laverty et al. demonstrated that their method of receptor reconstitution preserved the physiological long-distance crosstalk between different ligand-binding sites: varying the amount of a given ligand bound to the receptor modulated the binding of a different, radioactively marked ligand to a distant site (Fig. 1f and Extended Data Fig. 2d of ref. 1).
Although it is possible that the co-purification of these mRNAs may have occurred due to unanticipated binding of a different RNA binding protein to the column, we find this less likely.
We then examined the binding of a different protein, fluorescently tagged gelatin, to the microposts.
Using too high a protein concentration increases the risk for nonspecific interactions or even binding of a different contaminating protein present in the preparation.
This study suggests that a high level of c-Myc may lead to the binding of a different set of target genes from those regulated by endogenous levels of c-Myc.
Recent work now demonstrates that fucose-containing ligands, such as those present on Helicobacter pylori, result in binding of a different set of proteins, IKKɛ kinase and the deubiquitinating enzyme CYLD, leading to nuclear accumulation of kinase Bcl3 and suppression of the proinflammatory response [ 41].
Similar(54)
Two monoclonal antibodies targeting the extracellular domain of ERBB2 receptor with different mechanisms of action, one binding to a different epitope and the other conjugated to a cytotoxin, have recently been approved for clinical use (Baselga et al, 2012).
Noncompetitive inhibition patterns can also be observed for active site binding inhibitors in two substrate systems when the inhibitor shows preferential binding for a different conformation of the enzyme than the acceptor substrate (Blat 2010).
Latrunculin B increases the level of actin monomers by binding to a different site on G-actin, the nucleotide-binding cleft, and does not interfere with binding at the hydrophobic cleft.
The trNOE distances determined for a bound LewisX conformation also differ significantly from the free conformation recently measured by solution NMR by Zierke et al. (Table 1), indicating preferred binding of a conformer different from the dominant solution conformer.
However, it is possible that certain erythroid-specific DHSs were excluded due to the presentation of similar DHSs as a result of binding by a different set of TFs in these non-erythroid cell lines.
More suggestions(15)
binding of a transcriptional
binding of a [4Fe-4S]
binding of a small
binding of a multimeric
binding of a few
binding of a monomeric
binding of a basic
binding of a ferrocene
binding of a molecular
binding of a certain
binding of a second
binding of a biotinylated-BH4-BCL2
binding of a rigid
binding of a nucleotide
binding of a large
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