Sentence examples for binding mutation into the from inspiring English sources
To further increase the modularity of the transcription factors, we also engineered a previously described DNA binding mutation into the DNA binding domain (DBD) of each chimera.
The system is controlled by introducing two point mutations into the full-length protein: one to abolish its ability to bind ligand (binding mutation) and one to decrease its thermodynamic stability (packing mutation).
To detect miRNA-binding sites, mutations into the 3′-UTR of EEF1A2 mRNA were introduced by the overlap extension PCR.
In fact, based upon this principle that we initially derived from the structures of anti-Dig and anti-Fluo antibodies, we originally introduced the 52+2 Cys mutation into the Biot binding antibody in a blind manner several months before having access to the structure that we generated for this antibody.
By introducing the D308A mutation into the predicted Ca2+-binding motif within the C-terminal domain of Sec62, we confirmed the function of Sec62 in regulating ER Ca2+ homeostasis.
Introduction of a double-point mutation into the NF-κB-binding site (TGGGGA to TGGG AC) to generate pGL-COX2-mκB was performed using the following (forward) primer: 5′-GGGGTACCGCAAATAATTTTTTATCAAACACTGTTTCTG-3′ (corresponding to a region from -907 to +70).
Finally, we have also redesigned a related switch, MBP317-347, to function as an OR gate for sucrose and maltose by introducing mutations into the binding site of the MPB domain that conferred sucrose binding without eliminating maltose binding.
The transcriptional activation of the rx2 pCRE through the predicted Sox2-binding site was severely attenuated by the introduction of mutations into the Sox-binding site (from 50.99 ± 2.19 to 9.93 ± 1.41) (Fig 6D).
To determine the functional significance of Spc105p binding to MTs it will be necessary introduce specific disrupting mutations into the putative MT-binding interface, a task that should be aided by ongoing crystallographic studies.
Thus, the introduction of mutations into the CaM binding site led to a faster exchange (in the complementation assay), meaning that those mutations perturbed the stability of the tetrameric assembly.
