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To find out the transcription factor partners of HIPPI we carried out enrichment analysis for TF binding motifs within a fixed 100 bp distance from HBS.
We found 9 out of 19 genes searched had conserved Foxa2 binding motifs within a 10 kb upstream and 5 kb downstream region around the transcriptional start site (Additional File 10).
The number of the identified YY1 binding motifs within a given DNA sequence corresponding to the transcript was further divided by the size of the mRNA sequence, yielding a YY1 density score.
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Experimental evidence typically included the presence of a consensus binding motif within a core promoter, in vitro binding assays and luciferase reporter assays.
Thus, evidently the conserved residues represent a binding motif within a linker between (Pfam) structured domains or in a disordered tail at the carboxy or amino terminus of a (Pfam) structured domain [ 99].
Excluding ChIP-seq peaks that did not correspond to any of these three CTCF binding motifs, we assigned each of the 9822 genes annotated by ENSEMBLE into a group corresponding to its nearest CTCF binding motif within a [−50kbp,+50kbp] region of its TSS, or, if none, as 'other genes'.
The identification of true binding motifs within large datasets is a challenging task for several reasons.
Although the ring topology of these compounds is quite different from other lipid II binding lantibiotics, these peptides appear to contain the mersacidin-like lipid II binding motif within their A-ring.
These results confirm that the unusual clustering of YY1 binding motifs within the OLFR genes is a feature of the mammalian lineage.
In the presence of chitin, two lysin binding motifs within the Ecp6 protein combine to produce a binding site with ultrahigh affinity for chitin.
About half of the members of the OLFR gene family have a range of 4-8 YY1 binding motifs within their protein-coding regions.
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