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The 1-kb promoters of these WRKY53-coregulated loci are enriched for the presence of W-box WRKY binding motifs when compared to the frequency distribution of all gene models across the rice genome (Fig. 3).
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Only four transcripts had additional motifs when compared with the reference protein sequences.
An Ets-binding site is the second most overrepresented motif when comparing Jurkat versus erythroid cells and is also overrepresented in Jurkat peaks versus control sequences.
KatA1 49 did not show any binding to Vn and, accordingly, KatA51 505 was not impaired in binding Vn when compared to the full-length protein (Fig. 5b).
Indeed, CSA 8M showed greatly increased binding to TCP1 when compared to CSA WT (Fig. 5c).
Removal of the C-terminus distal of HBD-3 (Vn80–373) had no significant effect on binding when compared to Vn80 396.
Thirty-two of the fifty enzymes exhibited some differences in the acceptor-binding site when compared to TM0415 (Supplementary Table 2).
This feature may explain their lack of motif significance when compared to the canonical core CTCF motif.
The putative APUM5 targets, DREB2A, RD22, COR15, and RAB18 contained 'UGUA' core binding motifs in the 3′ UTRs when compared with the hbNRE2 motif.
Furthermore, we observed a less than a 1.1-fold difference in the median fasting/re-fed CREB binding ratio when comparing subsets of CREB sites separated by CRE motif occurrence, further contradicting the model proposed by Nichols and colleagues [ 7].
Interestingly, in a recent comparative study of fixed inverted differences between the genomes of humans and chimpanzees, Farré et al. (2013) have proposed that the lower recombination activity observed within inverted segments was linked to a lower density of PRDM9 binding motifs found within these regions, when compared with collinear regions on the same chromosome.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com