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The binding motifs were considered significantly overrepresented in a module if Clover p-value was lower than 0.01.
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For this, only transcription factors with binding sites that had less than 1,000 possible variants of their cognate binding motif were considered.
Loci without either binding motif are considered recessive lethal, and have a fitness of zero.
Motifs with FIMO p-value ≤0.001 (short motifs) or ≤0.0001 (longer motifs) were considered significant.
The proteins of groups B and D also contain the RExxxH motif and the RxxxxR motif, respectively, and both these motifs are considered to be CRP-like DNA-binding motifs (Vega-Palas et al. 1992; Maghnouj et al. 2000).
This motif is considered as a velogenic motif according to OIE [3].
DNA binding motifs were predicted by PlantCARE [ 60].
The binding motifs were obtained from Gene Regulation and PlantCARE [ 63].
NAD and ADP were observed in a subset of RCK domains that contain the conserved nucleotide-binding motif and were considered as the ligand for the RCK-containing K+ transporter.
Since one ligand atom can belong to more than one fragment in this definition, the patterns of the interactions in larger parts of molecules, i.e. those derived from binding motifs, can be considered by focusing on the consensus of the fragment interactions through atoms that are shared by more than one fragment.
The RbsR_EC and RbsR_PP groups represent two groups of ribose repressors from different bacterial lineages (Enterobacteriales, Vibrionales and Pasteurellales, and Pseudomonadales, respectively) that share the ligand specificity but have different DNA binding motifs, thus being considered as separate groups in our study.
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