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It was shown here that at least four IMMAD sequences relate to specific binding motifs of human RNA-binding proteins that have homologs in C. elegans.
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Such an effect was confirmed by Zuurbier's laboratory (Smeele et al., 2011) using a cell-permeable peptide of HK2 binding domain (TAT-HK2) whose sequence corresponds to the mitochondrial binding motif of human HK2 and which others had shown causes HK2 dissociation from mitochondria (Gelb et al., 1992; Chiara et al., 2008).
After processing, we obtained 177 binding motifs of yeast, 158 of human and 148 of mouse, respectively.
As Tip-1 has also been shown to bind to the carboxyl terminal PDZ binding motif of the human T-cell leukaemia virus (HTLV1) Tax oncoprotein (Rousset et al, 1998), there is good evidence that Tip-1 may have a role in viral carcinogenesis.
A recent analysis mapped DNAse1 sensitivity sites that harbor binding motifs of various TFs throughout the human mtDNA (Mercer et al. 2011).
(A ) The conserved binding motifs of TRAF2, TRAF5, and TRAF6 in human MAVS.
Fig. 2 Identification of binding motifs of recombinant OsSMF1 from E. coli by Q9-PBM.
Here, we used the Q9-PBM to determine the binding motifs of OsSMF1.
We first attempted to identify the binding motifs of the OsSMF1 protein using a Q9-PBM.
It can detect alternative binding motifs as well as binding motifs of potential co-factors.
The high degree of homology between the two RNA-binding motifs of Drosophila TBPH and human TDP-43 and the finding that they can functionally substitute for each other (Feiguin et al. 2009; Lu et al. 2009; Li et al. 2010) suggest that the target binding sequence is also conserved.
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