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The method is based on incorporation of structurally non‐perturbing, specific binding motifs for a bis‐arsenical fluoroscein dye, FlAsH, in sites that result in distinct dye fluorescence signals for the folded and unfolded states of the protein under study.
"Epitope repertoire" here refers to all viral peptide sequences that fulfill HLA class I allele-specific binding motifs for a specific whole HIV-1 proteome.
The C domain contains binding motifs for a Rieske center and non-heme iron and catalyzes the conversion of chlorophyll a to chlorophyll b [ 4, 8, 9].
Instead, binding motifs for a large number of other transcription factors were identified in the upstream regulatory regions of these genes [ 23].
This observation is in agreement with our findings that BRG1 silencing affects expression of many more genes than MITF and that the binding motifs for a variety of factors other than MITF are enriched at BRG1-occupied sites.
Moreover, the binding motifs for a variety of factors are enriched at BRG1-occupied sites suggesting that it can be recruited to the genome of melanoma cells by many other transcription factors.
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Accessible regions were analyzed to reveal enrichment of a binding motif for a certain transcription factor involved in disease (prostate cancer).
An Arg-Gly-Asp (RGD) binding motif for a subgroup of integrin adhesion receptors (reviewed in [ 44]) is found in TGFBI exon 15, which is followed by two short exons 16 and terminal exon 17.
Systematic evolution of ligands by exponential enrichment (SELEX), also known as in vitro selection, is a common method for determining the consensus binding motif for an RBP [ 62, 63].
In addition to the Hsf1 binding sites, MEME identified a possible binding motif for an AP-2alphaA-like transcription factor in 11 of the 64 differentially regulated proteins (AFUA_1G05610, AFUA_4G07710, AFUA_1G10130, AFUA_5G04170, AFUA_5G07340, AFUA_2G17110, AFUA_2G10660, AFUA_3G09320, AFUA_6G12660, AFUA_1G02030, AFUA_7G01860; see Figure 4 for motif logo).
The presence of consensus binding motifs for RAX, an rx3 ortholog in humans, was sought in 5 kb promoter regions of selected zebrafish genes.
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