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Both D. willistoni mtrm genes show a Polo-box domain binding motif and a typical Plk-phosphorylation motif in the same protein region as in D. melanogaster (Table 2).
When DegPS210A was incubated in the presence of the P+Z+ peptide containing both a protease binding motif and a PDZ1 binding motif, the enzyme eluted as a 12-mer oligomeric cage upon injection into the filtration column (Fig. 4B), consistent with rearrangement in the presence of small substrates.
SBP2L has an L7Ae RNA binding motif and a domain with clear sequence homology to the SID.
Thus, for these two HTCS paralogs, we also proposed the existence of one DNA binding motif and a joint regulon, named HTCS_Rgu-2.
Regarding DNA damage repair, we identified SigC as a key control element, 12 new targets for LexA, an updated LexA binding motif, and a potential mismatch repair system.
All Neph proteins consist of five extracellular Ig-like domains, a transmembrane domain, and a cytoplasmatic tail that contains a podocin-binding motif, a PDZ binding motif, and a SH2-binding site (Sellin et al. 2003).
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The complete RHS copies (n=38) have characteristic features of the family, such as the ATP/GTP binding motif and an insertion site for retrotransposons (Additional file 5).
The PTEN-related protein, TPTE, is very similar in sequence to PTEN through the phosphatase and C2 domains, but lacks a PDZ binding motif and an extensively phosphorylated C-terminal tail.
The A4GALT expansion consists of 3 complete tandem copies; all contain a glycosyltransferase DXD-sugar binding motif and an alpha 1,4-glycosyltransferase domain, the latter involved in protein glycosylation.
Structural motifs characteristic of vertebrate neuroligins are generally present in the honeybee molecules; namely, a signal peptide, cholinesterase-like domain, EF-hand metal binding motifs and a trans-membrane domain (Figure S2).
On the other hand, no apparent effect is observed on the expression of the two major cold-shock induced proteins CIRP and RBM3, which like nucleolin contain RNA binding motifs and a C-terminal glycine rich domain, and are up-regulated in human tumors [3], [49].
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