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The association of the Vch_cass2 gene with mobile DNA elements, also notably evident for its group of related homologs, emphasises the mechanism by which these binding modules can be laterally transferred between species.
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A peptoid backbone was chosen as the modular display scaffold because (i) valency and the distance between RNA-binding modules can be easily controlled, (ii) their synthesis is facile and various RNA-binding modules can be easily incorporated, and (iii) they are cell-permeable.
Recent examples also illustrate how a carbohydrate-binding module can effectively extend the active site of a hydrolase [ 40] and that PA14 carbohydrate-binding modules can be inserted into the hydrolase domains rather than just being appended to them [ 41,42].
Enhancers and cis-regulatory modules can be predicted by clustering predicted binding sites, an approach taken by programs such as Stubb [19], [20], eCis-Analyst [21], [22] and Cluster-Buster [23].
Upon interaction with their cognate binding sites, transcription factors mediate DNA looping so that even distant modules can be brought to interact with the basal transcriptional apparatus [9].
Simple motions of the modules can be combined and sequenced.
Single modules can be completed over a weekend, priced from £190 per person.
And the mix of modules can be tuned precisely to an individual's interests and needs".
The modules can be flexibly arranged.
Modules can be combined together in any order, but are cloned sequentially one module at a time to form a composite module, which can then be further subcloned.
Specifically, the black module can be considered a pluripotency/self-renewal module, while the blue, brown, and cyan modules can be considered differentiation modules in the signed network.
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