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The titration curves were analyzed with Origin X (MicroCal) with ones-site binding model, with the first injection point excluded.
Data were plotted and analyzed using a single site binding model with the MicroCal Origin software.
The sensorgrams were corrected against a buffer only reference and fit with the ForteBio Data Analysis package assuming a 1 1 Langmuir binding model with the global fitting function.
Analysis with a two-site binding model with the assumption that the binding at each site generates the same change in CD signal improved the χ value to 1.2.
Binding affinities were determined by globally fitting the chemical-shift changes of the protein-backbone resonances (both H and N) as a function of the molar ratio of RNA-to-protein for each titration point using a two-site binding model with the program nmrKd2.
The conserved physical pattern can be rationalized in terms of a qualitative binding model with the catalytic cleft of the protein kinase A. Positions -6...+4 surrounding the phosphorylation site are influenced by direct interaction with the kinase in a varying degree.
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We describe the structure of the AGNR with an embedded line defect using the tight-binding model with the nearest-neighbor approximation, i.e.: H = H C + H D + H T, (1).
The structural and optical properties of CdSe/ZnS core/shell nanocrystals are studied using a combination of valence force field and sp3s⁎ empirical tight-binding model with the aim of studying the relative impacts of ratios between core and shell radius in the fixed volume.
It is shown, in the orthogonal π-orbital tight-binding model with the nearest neighbor approximation, that if the ribbon width is a half of the tube circumference the effect takes place for all achiral ribbons (zigzag, armchair and bearded), and corresponding tubes, starting from lengths of about 30 nm.
Equation 3 can be transformed into a convenient form in which Ptot is substituted with the ratio Ptot n H/ Otot as the independent variable to yield 4Equation 4 reduces to the single-site (Langmuir) binding model with modifications explicitly considering the total DNA concentration where nH = 1.
Data were analyzed using a one-site binding model with Origin ITC Analysis software provided with the instrument.
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