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(1) In mixed zwitterionic/anionic vesicles the charge effect on the protein binding model was analyzed.
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The BIA evaluation software (GE Healthcare) assuming the Langmuir 1 1 binding model was used to analyze the data.
The Kd values are listed in Table 2 and the fits are shown in Figure 2. The Kd values obtained from the two-state binding model were statistically analyzed using an F-test and Akaike's test of the DOF/sum of squares.
28 The similarities and differences between the models were analyzed in detail with regards to experimental data on agonist affinity, efficacy, and effects of binding site mutations.
All generated models were analyzed with Chimera 1.10.1 software68.
Three models were analyzed.
Three adjusted models were analyzed.
Two multivariate models were analyzed.
For GR DBD binding to κBREs, a two-site binding model was used.
The corresponding tight binding model is also constructed.
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