Sentence examples for binding model using the from inspiring English sources

The saturation binding data was analyzed with nonlinear regression assuming the one site specific binding model using the equation Y = Bmax*X/ Kd+X) using GraphPad Prism (GraphPad Software Inc. San Diego, USA). 1 mg/mL rCR2 and K41E CR2 were labeled with 400 MBq [99mTc(CO)3(OH2)3]+ for 30 min. at 37°C with gentle shaking.

Each titration was fitted to a simple one-site binding model using the Origin software provided with the instrument.

The derived curves were fitted to a 1 1 Langmuir binding model using the biacore evaluation software.

CSPs (> 0.1 ppm) were fitted to a 1 1 binding model using the programme KaleidaGraph to derive an apparent Kobs.

Affinities were determined by fitting the heat changes to a one-site binding model using the associated Origin package.

Data were fitted to a one-site binding model using the Origin 7 Software (MicroCal) to obtain stoichiometry (N), enthalpy (ΔH), entropy (ΔS) and association rate constant (Ka).

Data were fitted to a one-site binding model using Origin 7. (E) The overlaid curves of apo RBBP4, apo AEBP2 ZF-RRK and mixture of RBBP4 with exccesive AEBP2 ZF-RRK by gel filtration assays on a same SuperdexTM 200 10/300 GL column.

The data were fitted to a 1 1 binding model using BIAevaluation 3.2. 1 nmole of the 12mer ribo-oligonucleotide was end labeled using γ-32P-ATP and T4 polynucleotide kinase with standard protocols.

The resulting isotherm was fit to a 1∶1 binding model using GraphPad Prism and showed the synbody had a Kd of 4.9±1.1 nM, in agreement with the SPR results (Figure 3C).

The data were fitted by nonlinear regression to a one-site binding model using GraphPad Prism (La Jolla, CA) software to obtain the affinity constant, KD from the following equation: Cortical neurons were dissociated with 1 mg/mL papain from embryos of GluN2B /– mice and plated on 35 mm tissue culture-treated dishes coated with poly- l-Lys.

Data was fit to a 1 1 Langmuir binding model using a single kinetic trace with the BIAevaluation software.