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The data was analyzed by curve fitting to a one-site binding model using a nonlinear regression.
The data were analyzed by curve fitting to a one-site binding model using a nonlinear regression.
Data was fit to a 1 1 Langmuir binding model using a single kinetic trace with the BIAevaluation software.
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After converting to heat per injection, the curves were fitted using a one-site binding model using Origin 8.1 software.
CSPs (> 0.1 ppm) were fitted to a 1 1 binding model using the programme KaleidaGraph to derive an apparent Kobs.
The resulting isotherm was fit to a 1∶1 binding model using GraphPad Prism and showed the synbody had a Kd of 4.9±1.1 nM, in agreement with the SPR results (Figure 3C).
Curve fitting was performed in Sigmaplot assuming a 1 1 binding model using the following equations for association and dissociation, respectively: y = a ∗ (1 − e − (k o b s ∗ t ) ) + y 0 ∗ t and y = y 0 + a ∗ e − (k o f f ∗ t ), where y0 is the constant to correct for baseline drift.
The data were fitted to a 1 1 binding model using BIAevaluation 3.2. 1 nmole of the 12mer ribo-oligonucleotide was end labeled using γ-32P-ATP and T4 polynucleotide kinase with standard protocols.
If applicable, band densities corresponding to free, singly and doubly bound nucleosomes were quantified using ImageJ software package and subsequently fitted together to a 2 1 binding model using in-house written MatLAB routine (MATLAB version 7.13.0, The MathWorks Inc., Natick, MA).
Thermodynamic data were analyzed with a single-site binding model using MicroCal PEAQ-ITC Analysis Software provided by the manufacturer.
Data were fit with a one-site binding model using Origin 7.0.
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