ITC isotherms at 30°C for both purified and mixed XOSs were readily analysed using standard binding models provided by the MicroCal/GE Healthcare, (Northampton, MA, USA) instrument manufacturer.
The equilibrium constant (KD) was determined by the 1∶1 Langmuir binding fitting model provided by the Biacore 3000 instrument software.
This prioritised binding model provides developers with a low-effort mechanism to manage data aggregation.
The data were fit to the 1∶1 binding with baseline drift model provided by BiaEval software.
Then the foreground gene expression data can be synthesized by a linear combination of motif-binding data and TF activities using a log-linear model provided by Liao et al. [ 8].
Event-based binding models provide simple unidirectional communication between software modules.
A possible explanation for the spatial restrictions of this sensitive Wnt3a binding site is provided by our model of the V337W substitution in Figure 6B.
Early useful suggestions of metal ions binding was provided by the old discipline of metal coordination chemistry by Werner [23].
The LooCI binding model [13] provides unreliable event-based binding using a decentralised publish-subscribe event bus communication medium.
In this paper, we propose Hitch Hiker 2.0, a component binding model that provides support for multi-hop data aggregation.
