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The binding data were analyzed with the 1 1 Langmuir binding model in the BIAevaluation software (BIAcore).
Differences in fluorescence polarization were fit using a single binding model in the program Origin (OriginLab, Northampton, MA, USA).
Prompted by this comment, we have re-evaluated fitting the Hill model to the binding data and used an alternative binding model (in the text and Figure 2d).
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The straightforward synthetic manners as well as the presumed binding model in this paper could be advantageous for further structural optimization for developing inhibitors against GH20 β-N-acetyl-d-hexosaminidases.
To further understand the above precursor mechanism, we now compare this to the conventional protein-receptor binding model in which the formation of the complex occurs in a single step: 8where ka and kd are the association and dissociation constants, respectively.
Ojamäe et al. suggested a binding model in which the adsorbate binds to surface titan atoms through its carboxylic end (by the formation of an oxygen bridge) and to a surface oxygen ion through its ammonium end (by an H bond).
Based on a successful atomistic tight-binding model in the conjunction with an empirical bowing parameter and the widely used virtual crystal approximation, the theoretical investigations of near infrared InAsxSb1−x nanocrystals with the experimentally synthesized sizes and As compositions (x) are reported.
It was not possible to obtain a good fit of the data shown in Figure 2 to a simple binding model in which the site was either occupied by fatty acid or empty (eqs 12 and 14).
We suggest you address the issues raised by this and the other reviewers by using the intermittent binding model in your simulations to determine the fit for the existing data on angular distribution.
Based on the constructed hDAT-Tat binding model in our previous work36, both the side chain and backbone of H547 forms a hydrogen bond with residue R49 of HIV-Tat (Fig. 1B).
Plotting of the change in shift of these hydrogen atoms against substrate concentration gave plots that fitted well to a 1 1 binding model in most instances and yielded the binding constants Ka listed in Table 1.
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