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However, this could not be examined further, as a two-compartment reversible binding model did not give stable parameter estimates.
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Two-site or cooperative binding models did not substantially improve the fits.
The diffusion-binding model did not lead to directional motion, indicating that diffusion of the ParB/ parS complex coupled with its biochemical interplay with DNA-bound ParA-ATP dimers does not create a Brownian ratchet.
Moreover, the diffusion-binding model did not work better than a simple diffusion model ('Materials and methods') in which the partition complex diffuses without interacting with ParA-ATP dimers.
As shown in Figure 4B and 4C, this diffusion-binding model does not lead to fast or robust segregation.
This can be seen in Video 2. As a result, the diffusion-binding model does not perform better than the model in which the partition complex only passively diffuses (no interaction with ParA-ATP dimers).
This model did not converge.
The model didn't show.
For simplicity, the model does not include binding of cross-linkers to regions flanking the midzone.
This modification of the model does not affect the binding mode of DOX.
The ionic screening model used here is Debye-Huckel and the model does not incorporate the possible effect of divalent binding.
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