In order to study this remarkable plasticity in molecular recognition in greater detail, we prepared a series of mutants designed to either knock out a specific binding mode or add length to the molecule at a specific position.
So far, under conditions in live cells, this contact seems to be weak in case of the MYA2 head-neck either indicating a flexible binding mode or that other factors might be involved such as the myosin tail or other cytoskeletal proteins.
DOI: http://dx.doi.org/10.7554/eLife.10319.004 To preferentially select for a specific binding mode (A or B), we prepared Doc sequences that incorporated 4 amino acid point mutations, referred to as quadruple mutants ('Q').
Binding residues 11, 12, 18, and 19 for binding mode A and 45, 46, 52, and 53 for binding mode B are shown as blue stick models.
In contrast, comparing the binding mode of FII-A to either that of lisinopril or RXPA380 reveals a different picture.
WAY-169916 is an unusual ERα ligand: It is able to bind ERα, leading to its constrained or unconstrained conformation (responsible for partial agonist activity, binding mode 1, or for an antagonist effect, binding mode 4, respectively) [ 23].
Baumgartner, R. et al. Dual binding mode of a novel series of DHODH inhibitors.
How much slack is there in associating a given binding mode with a PRE signal.
As such, it helps to get a better understanding of the binding mode of an inhibitor.
The difference of the binding mode led to a difference of the phosphorylated position of the produced myo-inositol monophosphate.
