Apoptosis was routinely assessed by the annexin V-binding method, which is based on the affinity of annexin V for phosphatidylserine externalised to the outer leaflet of the plasma membrane early in the course of opoptosis.
Apoptosis was routinely assessed by the annexin V-binding method, which is based on affinity of annexin V to phosphatidylserine externalised to the outer leaflet of the plasma membrane early in the course of apoptosis (Neuzil et al, 2001a).
The QM method used in our studies was the self-consistent charge density functional tight binding (SCC-DFTB) method, which is a semiempirical model that combines good accuracy with relatively fast computational performance.
In the present study, we have succeeded in the development of a chemical-reaction-dynamics simulator based on our original, tight-binding, quantum-chemical, molecular-dynamics method, which is more than 5000 times faster than the regular first-principles, molecular-dynamics method.
Therefore, our method, which is based on the continuous miRNA-target binding score data, achieves more accurate results than those methods based on miRNA target gene set analysis.
In addition, the blind test in the DREAM4 project motivated us to develop a method which was not dependent on the canonical binding motifs.
For example, the ligand binding method, which has been held to be a sensitive method for measurement of EGFR, measures functional properties and the technique concentrates on membranous proteins only (Yoshida et al, 1997).
This review summarizes the common analytical methods which are used to study the active herbal components-protein binding and gives the examples to illustrate their application.
Here we report a new binding site prediction method PocketDepth, which is geometry based and uses a depth based clustering.
The use of multiple crown ethers is shown to be an efficient method for enhancing the binding energy, which is a critical factor influencing the success of these reagents.
All binding values are the result of fitting titration data to a 1 : 1 binding model, which is consistent with Job's method of continuous variation.
