Sentence examples for binding method as previously from inspiring English sources

At the end of the 21-day alginate culture period the medium was removed, and the alginate beads were collected and processed for PG assays using the DMMB binding method, as previously described [ 25].

ACAN, aggrecan core protein; ADAMTS5, a disintegrin and metalloproteinase with a thrombospondin type 1 motif 5; FGFR, fibroblast growth factor receptor; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; MMP13, matrix metalloproteinase-13. Cultured cells on alginate beads were collected and processed for quantitative assays using the DMMB binding method, as previously described [ 26, 27].

Screening of TAs that binds to recombinant E-selectin protein was carried out using a solution-based filter binding method as described previously [24].

GTPγS binding was measured using a filter binding method as described previously (Garcia-Marcos et al., 2010, 2011b).

Prior to ligand binding experiments, protein concentrations were determined by the Lowry method as previously described, using bovine serum albumin (BSA) as the standard.

The concentration of the latter compounds required to inhibit the binding between MDM2 and p53 by 50% (IC50 values) was determined using an ELISA-based method as previously described (Hardcastle et al, 2005).

From 1945 to 1982 we used the_ manual method the hand searching method as previously described [5].

The methods were as previously used (Yan et al., 2010).

Analytical methods were as previously described [33].

Laboratory methods were as previously detailed.

The methods were as previously described (19).