Serum and urinary Calcium was measured by the colorimetric cresolphthalein binding method, and phosphorus was measured by the phosphomolybdate ascorbic acid method [32].
Serum urea nitrogen (BUN) was determined using a BUN diagnostic kit from Pointe Scientific, Inc. Serum calcium (Ca) was measured by the colorimetric cresolphthalein binding method, and phosphorus (P) was measured by the phosphomolybdate ascorbic acid method (Stanbio Laboratory, TX, USA).
Viral RNA was isolated using the QIAamp Viral RNA Kit (Qiagen) silica membrane binding method, and the protease and RT genes were amplified in seven different RT-PCR reactions.
For example, the ligand binding method, which has been held to be a sensitive method for measurement of EGFR, measures functional properties and the technique concentrates on membranous proteins only (Yoshida et al, 1997).
Atomistic simulations based on the density functional based tight binding method (DFTB) are performed on a (9, 6) SWCNT and the simulation data is used to estimate material parameters of this rod model.
In the live cell binding method, the cells were incubated with peptides in cell growth medium for two hours at 37°C.
This receptor binding method was adapted from previous studies (Jones and Wonnacott, 2004; Oddo et al., 2005; Shelukhina et al., 2009).
The total enzyme protein determined according to the Coomassie Blue binding method was 2.2 mg/mL.
The dye binding method was used to measure the protein contents of the samples as described by Bradford [ 13] using BSA as the standard.
Apoptosis was routinely assessed by the annexin V-binding method, which is based on the affinity of annexin V for phosphatidylserine externalised to the outer leaflet of the plasma membrane early in the course of opoptosis.
