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In both studies, the authors provide binding measurements for each factor to all approximately 6000 yeast intergenic regions.
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Following binding measurements, Cl- concentration for each sample was assessed using a CMT19 chloride titrator (Radiometer, Copenhagen, Denmark), and pH measured in oxygenated sub-samples equilibrated to experimental temperatures (25 and 37°C) using a Radiometer BMS2 Mk2 Blood Micro system and PHM 64 Research pH meter.
The microcalorimeter can also be used for binding measurements.
The microcalorimeter employed can be used for binding measurements.
Importantly, HAc was measured in LPS-stimulated macrophages, consistent with the conditions for TF binding measurements.
Binding measurements were acquired in triplicate for each DNA substrate.
Furthermore, the identification of aptamer pairs remains a low-throughput process because binding measurements must be performed serially for every combination of potential binding pairs.
These results showed that for blocked binding measurements ≤85% of baseline, statistical significance could be achieved with ≤3 replicates, a feasible group size for preclinical imaging throughput.
Equilibrium binding curve for ABA1; control experiments for aptamer pair binding; contribution of linker length to binding affinity for bidentate aptamer reagents; binding affinity measurement for ABA1-ABA65 using ELONA; binding affinity measurements for control sequences; table of aptamer array features; table of screened aptamers, bivalent aptamer reagent constructs and control sequences.
There is no clear explanation for such significant discrepancy between the binding measurements by FTSA-ITC and inhibition of activity measurements by stopped-flow CO2 hydration assay.
(C ) Fluorescence anisotropy binding measurements showing competition between wild type rhodamine-VCA and unlabeled VCA constructs for actin monomers.
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