It seems probable that a PRE is more likely to be determined by the presence of multiple binding sites for an array of DNA-binding proteins, and that Pho binding may involve cooperative interactions with some combination of other DNA binding factors (Kassis and Brown 2013).
It should be noted, however, that adsorption and binding may involve mechanisms other than cation exchange.
Furthermore, the relatively modest affinity of single UIM for monoUb leaves open the possibility that the mechanism of high-affinity binding may involve cooperation between multiple UIMs [14], [15], [45] and polyUb chains [46], [47].
However, FgnE lacks these domains, so FgnE binding may involve one or more novel, non-RGD sites.
These observations suggest that C2AB membrane binding may involve some sort of favorable thermodynamic interaction between the two domains, which would allow the C2AB tandem to overcome barriers to deep membrane insertion experienced by individual domains.
The bundling or crosslinking mechanism of sAB-27 is unclear, but may involve an extended binding-site that can bridge two closely-spaced actin filaments.
Therefore the supralinear calcium release cascade initiated by IP3 binding IP3R1 may involve cytosolic calcium also binding RYR to increase calcium release from the sER.
Although the mode of action of sPLA2 in this animal model is not exactly known, the potential mechanism may involve binding to a receptor [ 39], followed by internalization [ 40] and transfer of sPLA2 to intracellular pools of phospholipids enriched in AA [ 41].
These agents produce their effects through various mechanisms, which may involve binding directly to the different α or β adrenoceptors or disrupting sympathetic activity peripherally or centrally.
The unique mechanism of neutralization by ADI-15946 may involve binding of ADI-15946 in the 310 pocket, which in turn prevents rearrangement of GP1 residues 71 75 on binding of GPCL to NPC1 by mimicking the hydrophobic interactions of the β17 β18 loop that packs into the pocket in GPFL (Fig. 3a,b)12.
