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The mechanism for the increased sensitivity results from increased binding mass and a gold plasmon coupling effect.
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The results showed that the dendrimer bound on TOCNF caused a saturation, while polyethyleneimines (PEIs) almost linearly increased the binding mass ratio per TOCNF as the mixing ratio rose.
The binding energy, mass and color coding are the same as in Figure4.
The two-site competitive binding model, including correction for PHNO mass on D3 binding and a small specific signal in the cerebellum, was applied to the measured data before and after motion correction.
ligand-binding mass spectrometry.
Hall et al. developed ligand-binding mass spectrometry (LBMS) for the detection of TMP fusion protein AMG531 (romiplostim) (Hall et al. 2010) that composed by TMP and the human Fc fragments as a typical "peptibody", in which those peptibodies were captured by anti-human Fc antibody for further Mass Spectrometry analysis.
Outline of the ligand isolation strategy, plasma fractionated by ion exchange that contains two SR-A binding peaks, and mass spectrometry identification of candidate ligands in SR-A binding bands from two similar purification runs (Table 1).
The two binding events were associated with a large mass change and a structural change component.
We observed a similar capability of the scFab polypeptides, resulting in a protein fraction with double the molecular mass and increased binding due to the avidity provided by two binding sites.
The conjugate was purified by HPLC and characterized by MALDI-TOF mass spectrometry and an I binding assay.
MsbA is a 'half-transporter', comprising a TM (transmembrane) domain with 6 membrane-spanning helices, which are believed to contain the substrate-binding site, and a NBD (nucleotide-binding domain), for a total molecular mass of 64.5 kDa [ 4– 7].
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