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The binding levels of the DARPin 57.2 were determined by the levels of cell surface CD4 expression.
Levels of Oil Red O staining were corrected for non-specific binding levels of the stain to untreated cells.
Moreover, such studies (or studies of whole embryos) do not allow direct comparison of expression effects at an individual gene level with binding levels of the MSL-complex.
The binding levels of the hTERT transcription factors SP1, c-Myc, and NF κB to its promoter region was assessed by the chromatin immunoprecipitation (ChIP) assay by using the ChIP kit (Millipore, Billerica, MA, USA) according to the manufacturer's instructions.
In the brain, these environmental contaminants caused neuronal degeneration in cerebral targets such as the mesencephalon and hypothalamus, damage that appeared to correlate with altered binding levels of the three major histamine receptors (subtypes 1, 2, and 3).
We then measured the effect of each of the derived variants by quantifying allele-specific binding levels of the MSL complex in F1 hybrids between the ancestral haplotype (GA) and each of the derived haplotypes (GT, TA, and TT).
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According to the attained results, the alumina-based binders (HA or ColAlu) induced a more effective sintering process due to their high specific surface area, improving the physical properties and the binding level of the generated microstructure.
As reported by Onunkwo [26], if the angle of repose decreased, the binding level of the granules increased.
The binding level of the analyzed aptamer pool after the second selection round was only about 2.1-fold higher when compared to the ssDNA library.
Again, the 3' part of the coding sequence of genes with an average binding level of the three MSL-proteins of about 0.6 is better predicted when compared to the 5' part.
This was due to the low binding level of the aptamer pool after the eighth SELEX round, selected with the help of zinc-coated agarose beads, to empty cobalt-coated agarose resin (data not shown).
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