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The β-Gal binding level was determined from the activity of alkaline phosphatase-conjugated strepavidin bound to the wells.
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Finally, the simulation runs for an additional 10 seconds during which time average binding levels are determined.
An alternative is the methodologically more challenging scan protocol consisting of a bolus-infusion administration of tracer, performed to achieve equilibrium, where control-binding levels are determined.
Peroxidase level was determined as described above.
This expression level is determined by the binding probability p α of the transcription factor.
IgA1 and glycosylation level were determined by enzyme-linked immunosorbent assay (ELISA) and VV lectin-binding method.
Thyroxin (T4), free T4, reverse triiodothyronin (rT3), triiodothyronin (T3), thyroid-stimulating hormone (TSH), and thyroid-binding globulin levels were determined in the umbilical cord blood of the 90 women, provided that enough cord blood was available to perform the analyses.
Serum lipopolysaccharide-binding protein (LBP) levels were determined with a commercially available Human LBP enzyme-linked immunosorbent assay (ELISA) kit (HyCult biotechnology b.v.; PB Uden, the Netherlands).
For nutritional assessment; albumin, prealbumin, retinol binding protein (RBP) and transferrin levels were determined.
Retinol binding protein-4 (RBP4) and resistin levels were determined by ELISA and RIA, respectively.
Adiponectin and retinol-binding protein-4 (RBP-4) levels were determined by ELISA (Adipogen, Seoul, Korea).
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