Sentence examples for binding lanes from inspiring English sources

A negative control DNA also did not compete for binding (lanes 7 and 8).

yPrp3CTF efficiently bound a yU4/U6 constructs bearing full-length stem II and yU6 3′-overhangs of at least eight nts, while further shortening of the yU6 3′-overhang led to progressively reduced binding (lanes 9 14).

In the presence of antibodies against NF-κB subunits p50, p52, p65, c-Rel, and RelB, the results revealed that the addition of an antibody against p50, p52 or p65 caused a substantial reduction in binding (lanes 7, 8 and 9).

In contrast, in TD cells, the lack of NF-Y binding (Figure 2A, lower panel, lane 2) correlates with the lack of RPII binding (lane 1) and the induction of H3K9me3 and H4K20me3 (lanes 5 and 6).

Incubation with a 100-fold molar excess of the unlabelled mutated probe did not alter binding to the wildtype probe (lane 3) but addition of unlabelled wildtype probe competed off all binding (lane 4).

The intensity of the DNA-protein complex was slightly depleted by c-Rel (lane 10) while antibody against RelB had no effect on binding (lane 11).

The sequence RD did not give a shifted band in the presence of PhoP under the experimental conditions, suggesting that the flanking sequences beyond the direct repeat are necessary for PhoP binding (lane 2 of Figure 2B).

Similarly, when ZNF274 or KAP1 ChIPs were sequentially immunoprecipitated with SETDB1 antibodies, there is specific enrichment of the ZNF180 binding site (lanes 4 and 7, respectively).

Furthermore, introduction of bulky Arg residues at the core (i.e., S1109 or I1208), but not the periphery (D1106), of the interface also resulted in the complete loss of binding capability (lanes 4 6).

As shown in Fig. 3A, a concentration-dependent mobility shift of SL3 probe was observed when increasing amounts of protein lysate derived from cells expressing GFP-L22 were added to binding reactions (lanes 3 5).

As expected, △1 behaved similarity to WT while the △2 and △3 mutants did not proceed past the initial binding location (lanes 6 and 8).