Sentence examples for binding kit from inspiring English sources

Unbound cells were washed away, and adherent cells were lysed and integrin binding function/expression was detected using a fluorescent DNA-binding dye provided with the integrin binding kit using a fluorescence plate reader (485/530 nm).

To detect apoptotic cells, the cellular location of phosphatidylserine (PS) and the cell membrane integrity/permeability of treated cells were assessed by staining and by applying Annexin V-FITC/PI binding kit, following the manufacturer's protocol (BD Pharmingen, USA).

Library construction and subsequent sequencing were performed using the SMRTbell Template Preparation Reagent Kit 1.0, DNA/Polymerase binding kit P4-C2, MagBead Kit and DNA Sequencing Kit 2.0 (all components supplied by Pacific Biosciences, Menlo Park CA, USA).

Apoptosis was evaluated by propidium iodide staining, according to the method of Nicoletti and colleagues [ 19], and by the binding of FITC-conjugated Annexin V, using the Apoptest binding kit, containing annexin V-FITC and binding buffer.

A ready-to-sequence SMRT bell-polymerase Complex was created using the P6 DNA/Polymerase binding kit 2.0 (Pacific Biosciences, Menlo Park, USA; p/n 100-236-500) according thethe manufacturer instructions.

SMRT sequencing was carried out on the Pacific Biosciences RS according to standard protocols, 4 SMRT cells with the C1 chemistry (diffusion loading, 1 × 90 min, 5 Kb fragment size) and 6 SMRT cells with the XL binding kit used in conjunction with the C2 sequencing kit (Magbead loading, 1 × 120 min, 20 Kb fragment size).

The binding of KIT ligand promotes KIT dimerization, and activates its intrinsic tyrosine kinase activity, thereby resulting in a transphosphorylation at several critical tyrosine residues and an activation of downstream signal transduction molecules (Blume-Jensen et al, 1991).

The prostaglandin E2 (PGE2) was assessed using a commercially available competitive binding radioimmunoassay kit (Prostaglandin E2 RIA kit, Perkin Elmer, MA, USA).

The binding of KIT ligand (KITLG) to the extracellular domain of KIT leads to the receptor dimerization, the intracellular autophosphorylation, and then tyrosine kinase activation (TKA) [ 9].

Transferrin saturation (TS) was measured using an iron and iron binding capacity kit (Sigma-Aldrich, Castle Hill, Australia).

A mechanism involves SCF binding to Kit that induces a rapid internalization of the ligand-receptor complex and its degradation through an ubiquitination process [28], [29].