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These systems utilize ligands that noncovalently and reversibly interact with the target protein, with release being tuned by both diffusion and the binding kinetics unique to the ligand−protein pair.
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Due to noticeable signal noise, colloid-induced signal deconvolution using binding kinetics appeared to be inaccurate.
To determine the binding kinetics of E36 to RNAs we bleached an entire myofiber and monitored the recovery over time.
The binding kinetics of Rsc9 to stress coding regions is similar to Hog1 (data not shown).
Antigen binding kinetics were comparable to those of the intact C6 antibody.
Thus, the binding kinetics has proven to be more rapid than previously believed.
The chapter accounts for improved pharmacodynamics and the application of binding kinetics to kinases.
We monitored the binding kinetics of GPM1 to gp96 or AIMP1 by surface plasmon resonance (SPR) using BIAcore 3000.
Further, the binding kinetics in addition to the dissociation kinetics indicated in Figure 3 may also be helpful.
We next analyzed the GU81 binding kinetics to VEGFR2 by fluorescence ELISA.
The fact that the negative exponentials fit better than the inhibitor binding kinetics is probably due to inhomogeneous binding kinetics extending over a larger concentration range.
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