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The similarity of compound 2a to 2b and 1 with respect to structure and binding strength, differing only in the linker to the acrylamide binding group, is further evidence that that it is the covalent binding property that determines the difference in binding kinetics, rather than e.g. a slow conformational change of the target enzymes.
In contrast to the nearly free diffusion of paxillin in the cytoplasm, the motion of paxillin near adhesions is slower and dominated by binding kinetics rather than diffusion.
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For the purpose of describing the tracer kinetics, rather than estimating the absolute parameters for binding or uptake, a region with low specific binding is mathematically appropriate for deriving the reference input.
These results show that these peptide micropatterns mainly impacted cell adhesion kinetics rather than cell proliferation.
We reasoned that characterizing temporal kinetics, rather than steady state behavior, may overcome this limitation.
The modeling approach used here differs from the classical approaches to modeling the phototransduction pathway by its focus on implementing each reaction in a fundamental form described by mass-action kinetics rather than via more abstract kinetics.
Therefore such experiments may in fact characterize the interference of two different stress responses with different induction times and kinetics rather than a bona fide oxidative stress response.
These phenotypic differences are likely to be caused by differences in how the organisms control metabolism through regulation and kinetics rather than by differences in metabolic network structure.
Previous reports have also pointed out that PCT kinetics, rather than the baseline or the peak values, correlate with patient outcome [ 28].
Y313 contributes more in the kinetics regime of arginine binding rather than in the regime of binding thermodynamics (affinity)—keeping the substrate in the active site sufficiently long for catalysis to take place (Fig. 6B).
This suggestion highlights the potential kinetics of miRNA regulation, in that changes in relative expression, rather than absolute expression, are a key regulatory determinant.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com