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In vitro, time-dependent cell uptake and internalisation showed slower binding kinetics for the PEGylated BN analogue.
In particular, we compare the binding kinetics for the probes immobilized on surface of the hemispherical and flat circular cells, the limit of thin slab of analyte solution over probe cell as well as hemispherical gel pads and cells printed in gel slab over a substrate.
The binding kinetics for the interactions of Png1p/Png1p-ΔH1 with denatured RNase B was analyzed by surface plasmon resonance (Fig. 4 and Table 1).
Obviously, the ultrasensitive response will be steeper the higher the difference in binding kinetics for the respective pathways is, i.e. the greater rk.
The sensorgrams measuring ternary complex formation clearly display both types of binding kinetics, the slow association and dissociation of the ligand to/from the immobilized type I receptor ectodomain and the fast binding kinetics for the interaction of the soluble type II receptor ectodomain with the preformed complex (Additional file 3).
Binding Kinetics for the MAbs and the data analysis was performed using BioRad ProteON manager software (version 2.0.1).
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As a result, it can lead to unique slow tight-binding kinetics for the β-hydroxymethyl chalcone in HDAC2 but not in HDAC1, and the inhibitory activity and selectivity are increased due to the longer target-resident time in HDAC2.
We determined the binding kinetics for variants of the dual-specific antibody bH1 and Herceptin as Fabs to immobilized VEGF (the receptor binding domain, VEGF8 109) or HER2 (the extracellular domain, ECD, of HER2) using surface plasmon resonance (SPR) measurements (Figure 2).
The binding kinetics for gD derived from the HSV-2 G strain were only slightly different from those for gp120 (Table 2).
The catecholamine binding kinetics for PheH R270K in the presence of phenylalanine were much simpler than those of the wild-type enzyme in the presence of the amino acid, resembling instead those of PheH Δ117 and the unactivated wild-type enzyme.
The importance of eliminating mass transport was also seen from comparison of the binding kinetics for CTB binding to GM1 measured by SHCS and an SPR study where the flow rate was such that data were collected under steady-state conditions.
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