Sentence examples for binding isotherms were then from inspiring English sources
Binding isotherms were then analyzed using BiaEvaluation 4.1 (Biacore AB).
A binding isotherm was then fitted to the data using a least squares calculation to yield a χ value.
A binding isotherm was then fitted to data and expressed as the heat change per mol of peptide against the peptide/protein ratio.
The binding isotherm was then fitted according to eq 4 (in ), which considers two interactive ligand binding sites per dimeric protein, according to the model shown in Scheme 2. The fitted parameters are summarized in Table 3.
The binding isotherms were fitted according to a model for a single set of identical binding sites by nonlinear squares analysis using Microcal Origin software.
The binding isotherms were analysed using GraphPad Prism.
MBD binding isotherms were calculated and analyzed as described below.
Direct binding isotherms were fit with the concentration of competitor ligand set to zero.
The resulting binding isotherms were analysed using standard equations assuming a 1 1 binding stoichiometry, as described earlier [ 17].
The binding isotherms were fitted using nonlinear regression with a one site binding module available in GraphPad Prism version 4.00 for Windows (GraphPad Software, San Diego, CA).
Heats of dilution were subtracted and binding isotherms were plotted and analysed with MicroCal origin version 7.0, assuming a single-site binding model.
