Sentence examples for binding isotherms were calculated from inspiring English sources

MBD binding isotherms were calculated and analyzed as described below.

The binding isotherm was calculated by integration of the thermogram using a single-site binding model (Origin version 7.0) to derive the three nonlinear parameters for the interactions: the association constant (Ka), the molar ratio (n), and the enthalpy change (Δ H).

The protein adsorption isotherms are calculated for flexible and rigid molecules as well as for mixtures.

Direct binding isotherms were fit with the concentration of competitor ligand set to zero.

The binding isotherms were fitted according to a model for a single set of identical binding sites by nonlinear squares analysis using Microcal Origin software.

The binding isotherms were analysed using GraphPad Prism.

The resulting binding isotherms were analysed using standard equations assuming a 1 1 binding stoichiometry, as described earlier [ 17].

The binding isotherms were fitted using nonlinear regression with a one site binding module available in GraphPad Prism version 4.00 for Windows (GraphPad Software, San Diego, CA).

Heats of dilution were subtracted and binding isotherms were plotted and analysed with MicroCal origin version 7.0, assuming a single-site binding model.

From the calculated c(s) traces of each experiment, different binding isotherms are available: (1) The overall signal average sedimentation coefficient, sw, from integration of both free and complex peaks of c(s), follows the corresponding signal average s value of species populations predicted from mass action law, irrespective of the reaction kinetics.

Representative data are shown in Figure  2; all other spectra and binding isotherms are provided in the Supporting Information.