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Immunoblotting of several TG2 mutants convincingly showed that GTP binding is unaffected for W241A (Gundemir and Johnson 2009).
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cdk binding is unaffected by these mutations, but dimerisation is altered.
Our results indicated that the rate constant for single nucleotide addition (kpol) was reduced 223 fold for the mutant enzyme relative to WT, but that nucleotide binding was unaffected.
Depletion of SR resulted in the loss of elongation arrest release activity, nascent chain targeting, secretory protein translocation, and membrane protein integration, although ribosome binding was unaffected.
In the striatum, nAChR binding was unaffected by parathion exposure.
In contrast, hippocampal HC3 binding was unaffected by parathion with or without a high-fat diet.
Intriguingly, binding was unaffected by removal of the BHD but completely disrupted when full-length p85 was in complex with RBD mutant p110β, strongly arguing for the RBD as the RAC/CDC42-binding site.
The remaining 85%% of families would be unaffected for now.
Collagen content and construct swelling were unaffected for all groups.
Such disorganization would be expected to promote massive merotelic attachments, assuming that kinetochore propensity for microtubule binding itself is unaffected.
The key finding in the paper is that the binding cavity for neuraminidase inhibitors is unaffected as suggested by molecular modeling.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com