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We show that HCV NS4B binds RNA and that this binding is specific for the 3' terminus of the negative strand of the viral genome with a dissociation constant (Kd) of approximately 3.4 nM.
Our results show that Cab45S binds to the NBD of GRP78/BiP, and this binding is specific for Cab45S but not other members of the CREC family such as Cab45G and RCN1.
The binding is specific for CXCR4 since it can be inhibited by AMD3100, a selective CXCR4 inhibitor.
We found that GFP-GAPA co-sedimented with Rac1a preferentially in lysates that had been treated with GTPγS indicating that the binding is specific for activated Rac1a (Figure 7C).
LEDGF/p75 binding is specific for lentiviral IN proteins (e. g. those of HIV, SIV, FIV, and EIAV [12], [19], [20], which makes it appealing as a candidate tethering factor since all the lentiviruses tested (HIV, SIV, FIV, and EIAV) show favored integration in active transcription units [5], [21] [32].
Temporal binding is specific for action – stimulus associations and does not occur when one stimulus predicts another.
Similar(52)
FP binding was specific for GPA, since its binding was completely inhibited by the TER-119 IgG, but not by an isotype control antibody (rat IgG2b).
Furthermore, none of these RNAs were isolated in the presence of only BAP (lane 4) demonstrating that the observed binding was specific for L22 and not an artifact of the BAP tag.
As expected, preferential binding with IgMa was observed: this binding was specific for the gp41 MPER 2F5 epitope, as the scrambled gp41 MPER peptide or a peptide corresponding to a region within gp41 MPER, but outside the 2F5 epitope (gp41 MPER HR1) exhibited little or no binding.
We also performed competition assays to verify whether protein complex binding was specific for the TCF consensus binding site.
Additionally the binding was specific for EGF since no other competitor than EGF was able to displace the binding of the labelled ligand from its receptor.
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