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Glutamine binding is seen to significantly reduce inter-domain motions about the hinge region.
By 60 min into the scan, nonspecific binding is seen to be cleared out in both studies and remains at stable levels.
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Both TPR1-CBD (Figure 8B, top panel) and TPR2-GST (bottom panel) bound Hsp90 in a nucleotide independent manner, but no binding was seen to CBD or GST alone.
In both cases, reproducible binding was seen to specific regions upstream of the starting codon.
Furthermore the kinetics of binding are seen to be strongly influenced by single amino acid substitution in the access channels.
The efficiency of binding site formation is seen to depend on two joint conditions: the binding site motif must be short enough and the promoter region must be long enough.
When the same structures are superimposed along the RNA backbone, the binding site of JAZ ZF3 is seen to vary between the structures: it is disordered with respect to rotation about the long axis of the duplex.
The distribution of average ∆∆G scores obtained through ABS-Scan analysis for residues in the binding site for decoy dataset is seen to be different from the native protein-ligand complexes.
However, when XAC-X-Texas Red (15) was monitored at the single-cell level, no specific binding was seen at concentrations up to 30 nM.
When XAC-X-TXR (15) was monitored at the single-cell level, no specific binding was seen at concentrations up to 30 nM.
As seen in the peripheral blood, no DARPin binding was seen at later time points.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com