In kidneys of Agtr1A mice, AT1-specific binding is virtually undetectable and renal AT1-specific binding is reduced by approximately 50% in Agtr1A heterozygotes.
For R28L, the CAP binding is reduced.
At 37 °C, binding was reduced by one order of magnitude to values of Δ f=−1.2 Hz and Δ D=0.09×10−6, that is, close to the detection limit of the instrument, in agreement with the small (less than 0.4%%) domain fraction observed at 37 °C.
At the proximal p53 binding site, HDAC1 binding was reduced after the heat shock and acetylated HDAC1 binding increased.
We also identified one case (mis-classified content word) in which a content word was treated as a stopword by our algorithm and erroneously removed (GO 0006328 'AT binding' was reduced to 110{GTERM}).
The binding of RNA Pol II was reduced at the coding region A, but not at the promoter, in the Ezh1 but not the Ezh2 knockdown, suggesting an impairment in the elongation step of the transcriptional process.
Of the nine date hypanthium-specific proteins, expression of two transcription-related proteins, GATA transcription factor 25 (spot 302) and inducer of C-repeat-binding factor expression 1 DNA-binding transcription activator (spot 302) (Table 1), was reduced at NTR and later increased at RIPE in comparison to S1 stage.
The mutations were engineered into Tld-HA and we first tested for Collagen IV binding, which is reduced by at least two-fold in all CUB2/3 point mutants.
This deviation from the neutral distribution, which reflects selection for functional binding sites, has a form suggesting a Malthusian fitness landscape that is peaked at nearly optimal binding, decreasing with negative curvature as the binding strength is reduced.
The risk of sequence artifacts due to interfering variants at primer binding sites is reduced through amplicon tiling in all but the terminal primers of gene regions.
