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However, a 50% reduction of BRCA1 230-534 binding is observed at a concentration where p53 is unable to bind (compare lane 4 and 8 in figure 5c).
The highest incidence of Ca2+ and Na+ binding is observed at a highly acidic pocket on the protein surface.
As expected, strongest binding is observed at physiological pH.
In the absence of L-Trp, zero or negligible binding is observed at 20 60 °C, whereas very weak levels of binding are seen at 10 °C, with an apparent Kd of 280 nM.
The binding of the LysM domain of the N-acetylmuramidase AcmD from L. lactis takes place at pH 4.0, which is close to its calculated pI of 4.3 (ExPASy) and no binding is observed at other pH conditions (pH 6.0 and 8.0) (Visweswaran et al. unpublished results).
For the 40 C-terminal residues, no lipid binding is observed at low lipid:αS ratios, but as previously observed for WT, considerable resonance attenuation is seen at high ratios, with distinct steps at locations that coincide with the positions of proline residues (at P120 and P128).
Similar(53)
Consistent with this, a version of the CaM-binding site peptide in which the equivalent of Ser has been replaced by a phosphoserine did not bind at all to CaM at pH 7.5, although some binding was observed at pH 6.5.
KLF4 and Sp5 binding was observed at both esBAF and GBAF binding sites, with slightly greater occupancy at GBAF complex binding sites.
In mature virions, in which NC is thought to coat the viral genomic RNA, NC binding was observed at specific sites that again included the 5′ UTR and the RRE.
Low levels of E2A binding were observed at several other lymphoid-restricted regulatory regions including the Ig heavy chain (IgH) intronic enhancer, the IgH 3' enhancers hs3b/hs4, the RAG-2 enhancer, and the 5' regions of the B29 and TdT loci.
No differences in initial binding were observed at 5 µl/min or above indicating no limitations to any combinations.
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