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This binding is not restricted to CD4+ cells but also includes CD8+ cells and B cells.
With such an approach, antibody binding is not restricted to specific epitope conformations: the epitope recognized by the IgM antibody need only be exposed within the oligomeric state.
To confirm that Z-VAD-FMK binding is not restricted to HS-treated parasites, we have analyzed the cpc null mutants using chemical and therapeutic CD inducers.
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Association of PDI1A with calreticulin was also observed in DTT-treated cells (see Additional file 1: Figure S4), implying that CRT-binding was not restricted to the oxidized form of PDI1A, which is noted for its higher affinity for unfolded proteins [ 17].
These studies indicate that expression of binding ligands to CXCR2 is not restricted to epithelial cells.
Addition of excess purified FH, FI or C4BP to several samples with high autoantibody titers diminished the signal (not shown) indicating specificity of the autoantibodies and demonstrating that the binding of these autoantibodies is not restricted to plastic absorbed protein.
One caveat is that since in aPKCΔN the Par-6 binding region is deleted, it is not restricted to the apical compartment through Par-6 binding, therefore the effect we observe may occur in another cellular compartment.
Sp1 has been shown to induce VEGF in an HIF-1-independent mechanism 11 and a strong association between Sp1 and gastric cancer has been reported 12. Interestingly, the Sp1-binding sites in VEGF promoter is not restricted for Sp1 alone as other transcription factors with similar DNA-binding motif, namely, Cys2-His2 type zinc finger, often bind to this promoter region 13.
So far, there is no structural information available on how Rpl11 is recognized by Syo1; however, binding studies revealed that Rpl11 binding to Syo1 is more complex and is not restricted to a linear motif as in the case of Rpl5.
IRS-1 is not restricted to binding to IR/IGF-IR but also has the capacity to interact with a variety of other proteins [ 21].
However, there is now increasing evidence that IRS-1 is not restricted to binding to IR/IGF-IR but is also capable of associating with a variety of other signalling-related proteins [ 17].
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