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Increasing ionic strength increases the off-rate (koff) more than 3-fold; presumably escalating it such that binding is not observed above 100 mM.
Notably, RAG1 binding is not observed at an intergenic region nor at a transcribed gene that lacks an RSS.
Notably, a large finger domain motion upon nucleotide binding is not observed in crystal structures of the Y-family DNA polymerases.
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Contrary to the in vitro result, in vivo specific binding was not observed in the rat brain using PET with [11C]MMPIP (Figure 4).
Although Mg2+ binding was not observed in our spectroscopic study, one can not completely rule out the possibility since the Trp fluorescence or CD only reflects the change on Trp residue(s) or the secondary content change, respectively.
NMB-1 binding was not observed in large diameter DRG neurons (Fig. 6d).
In contrast, ESTA-1 binding was not observed in the vessels in the normal counterpart.
In contrast, binding was not observed when ixolaris was used as the control analyte (data not shown).
This reduction in binding was not observed when soluble 2 was incubated with the serum.
In contrast, dose-dependent binding was not observed in cells incubated with free I+A33-scFv-Fc.
However, the conformational change in the fingers domain expected upon nucleotide binding was not observed for any of the soaks.
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