H1 binding is not sequence specific; however, it shows binding preferences for certain DNA sequences.
Consistent with genome-wide binding studies for other cohesin subunits [24], [28], inspection of the bound sequences did not reveal a RAD21 specific consensus sequence, indicating that the cohesin complex is not a sequence specific DNA binding complex.
These results showed that the observed FORCA-binding activity is DNA-sequence specific and that only the TGGGC sequence of FORCA and not sequences flanking this invariant core are critical for this interaction.
The interpretation was that zinc fingers 3 and 4 were not required for sequence-specific DNA binding.
This binding is probably not specific to any particular target DNA sequence.
These results are consistent with DNA sequence-specific binding of AhR to XRE-related motifs.
Diversity of biological function of p53 family members is not reflected in differences in sequence-specific DNA binding.
A mutant oligonucleotide abolished AR binding, indicating that the in vitro DNA-AR binding is sequence specific.
Direct measurement of the binding affinity between a TF and DNA sequence is not easy.
It is not known if there is a specific DNA sequence or structure that is needed for DNA silencing.
