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In contrast, the fluorescence at the tips does not increase with the total fluorescence of the fibre (green circles in Figure 3A), indicating that tip binding is limited by the presence of a limited number of binding sites that easily saturate.
Eukaryotic gene expression is often under the control of cooperatively acting transcription factors whose binding is limited by structural constraints.
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Non-specific binding was limited by incubation with 0.25 μg of anti-CD16/CD32 anti-CD16/CD32ntibody 2.4G2, eBioscience, San Diego, CaliFcrnia, USA) per 1 million cells in 25 μl for 15 minutes.
The definition of transcriptional networks through measurements of changes in gene expression profiles and mapping of transcription factor binding sites is limited by the moderate overlap between binding and gene expression changes and the inability to directly measure global nuclear transcription (coined "transactome").
The problems solved concern two enzyme-catalyzed reaction systems that obey diversely modified Michaelis Menten rate equations, and biomolecule surface binding that is limited by mass transport.
Our bioinformatic search for transcription factor binding sites is limited by the fact that it will not detect regulatory sites where recruitment to the DNA is dependent on tethering to other transcriptional cofactor(s), a mechanism that is estimated to account for 30% of nuclear receptor-dependent gene regulation [2].
However, the Kiexp calculation derived from Equation 3 has taken into consideration that the range of inhibitor potencies resolved in fluorescence polarization-based binding assay is limited by the affinity of the fluorescent ligand.
To evaluate whether SM binding efficiency is limited by on- or off-rates, SNARE Sec17 Sly1 co-complexes were formed at 30°C for 60 min, washed in either 30°C buffer or 4°C buffer, and incubated an additional 60 min at the same temperature as the prior wash.
This means that the surface binding of –COOH of methyl red is limited by its stability, resulting into insufficient dye adsorption because of the acidic nature of the dye [24].
(C ) At 1 μM ATP the process is limited by ATP binding.
However, TF binding analyses based on tiling arrays is limited by the extent of probe coverage.
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