The length increase observed upon reduction of Dync1h1 is striking since this ATP-binding subunit is indispensable for all dynein-based axonal transport processes.
Chitin binding to AtLYK5 is indispensable for chitin-induced AtCERK1 phosphorylation.
However, others have reported that the C-terminal region of TNFR2 responsible for the binding of TRAF2 is indispensable for signal transduction and NF κB activation [ 41].
Furthermore, vertebrate NPC2 has a similar binding fold as MD-2 (a lipopolysaccharide-binding protein), which is indispensable for toll-like (TLR4) receptor signaling in the vertebrate immune system [ 55].
However, many of the prerequisites to AKT phosphorylation have not been fully clarified, i.e., whether PtdInsP3 binding or membrane localization is indispensable for AKT phosphorylation by PDK1.
Although the trapped cells can be easily applied for the facile supply and retrieval of media, reagents, and other chemicals, the lack of extracellular matrix that is indispensable for binding, interaction, and proliferation of cells causes a different behavior of the cultured cell compared with in vivo state.
In class I synthase family members, these motifs are involved in the binding of Mg2+ ions, which is indispensable for substrate recognition.
We found that the RING domain, though not directly involved in UHRF1 chromatin binding or interaction with DNMT1, is indispensable for DNA methylation by DNMT1.
In the present work, we identify HDAC2 as a key subunit which bridges the binding of APPL1 to NuRD and is indispensable for this association.
The sequences of all target sites (22 nucleotides or 23 nucleotides) begin with GG at the 5' end because transcription is initiated from GG bases and ended with the protospacer adjacent motif (PAM NGGG at the 3' end, which is indispensable for Cas9 binding and cleavage [ 27].
